Effects Of E-Cigarettes On Allergic Airway Disease
Flavored e-cigarettes are preferred by the majority of users yet their potential toxicity is unknown. Therefore our aim was to determine the effect of selected flavored e-cigarettes, with or without nicotine, on allergic airways disease in mice. To determine if any effects were due to nicotine, we compared the effects of flavored e-cigarettes with and without nicotine at 12mg/ML. Mice were exposed to indoor air and e-cigarette fumes twice a day for 30 minutes, six days a week.
Effect of e-cigarette exposure on weight
The effect of e-cigarette exposure on weight gain was assessed by measuring weight on Day 21 before the assessment of lung function. There was no effect of HDM on weight in female or male mice exposed to the e-cigarette with or without nicotine. male mice exposed to PG/VG with nicotine had reduced weight compared to Room Air exposed mice.
Effect of e-cigarette exposure on HDM-induced airway inflammation
e-cigarettes without nicotine
macrophages were increased in HDM mice exposed Black Licorice compared to Room Air, with a trend towards increased total leukocytes. PG/VG, Kola or Banana Pudding did not alter total leukocytes, eosinophils or macrophages. there was no difference in neutrophils between HDM mice exposed to Room Air and those exposed to any of the nicotine-free e-cigarettes. This suggests that flavored e-cigarettes without nicotine have variable effects on airway inflammation during allergic airways disease, with the direction of the effect and the type of inflammatory cells affected dependent upon the specific flavor.
e-cigarettes with nicotine
all e-cigarettes containing 12 mg/mL nicotine dampened airway inflammation. Compared to HDM mice exposed to Room Air, mice exposed to PG/VG and all flavored e-cigarettes had reduced total leukocytes, eosinophils, and macrophages. the present findings suggest that the effect of flavored e-cigarettes without nicotine on HDM-induced airway inflammation is dependent upon the specific flavor. In contrast, nicotine universally suppressed airway inflammation regardless of the flavor.
Effect of e-cigarettes on HDM-induced airway hyperresponsiveness
In mice exposed to e-cigarettes without nicotine or with nicotine, HDM treatment increased AHR as measured by Rrs, Ers, Rn, G, and H, There was no difference between HDM treated mice exposed to Room Air and any of the e-cigarette exposures as measured by Rrs, Ers, Rn.
Effect of e-cigarette exposure on HDM-induced airway remodeling
In mice exposed to nicotine-free e-cigarettes, HDM treatment increased soluble content. In mice exposed to 12 mg/mL nicotine, soluble lung collagen was increased with HDM treatment but there was no difference between mice exposed to Room Air and any of the e-cigarettes.
there was a consistent suppression of airway inflammation without any effect on AHR or airway remodeling due to e-cigarettes with nicotine, regardless of the flavor. Flavored e-cigarettes without nicotine had significant but heterogeneous effects on features of allergic airways disease. This suggests that some flavored e-cigarettes may alter asthma pathophysiology even when used without nicotine.